|Tokurahu, K., Okamoto, S., Katsuki, M., Nakagawa, H. and
Kotani, S. (2001) The actin-depolymerizing factor destrin has an actin
stabilizing domain. Biochem.Cell Biol. 79, 773-778.
|Destrin is a 19 kDa actin-depolymerizing protein of the
ADF-cofilin family. Destrin was digested with trypsin to a structurally
stable 9.2 kDa fragment that contains the actin-binding sequence .
The purified 9.2 kDa fragment has an actin filament stabilizing
activity, rather than an actin filament depolymerizing activity.
The deleted region is probably essential for the actin filament
depolymerizing activity of intact destrin. Surprisingly, the 9.2 kDa
fragment also has an assembly-promoting activity in the presence of ATP.