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Pathogenic E.coli and the cytoskeleton

Pathogenic E.coli adopt one of two main strategies in order to parasitize the animal host.  E.coli can form tight adhesions with the cell surface or the bacteria can force the cell to take it up by phagocytosis. 
Figure 1. EHEC are thought to use cell surface nucleolin as a receptor (Sinclair & O'Brien, 2002).
  Bettelheim & Thomas Birmingham, UK
E.coli as pathogens
  John Leong Univ.Mass Med.School US Food & Drug
  Brett Finlay's pages EPEC EHEC

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Cantarelli, V. V., Takahashi, A., Akeda, Y., Nagayama, K. & Honda, T. (2000) Interaction of enteropathogenic or enterohemorrhagic Escherichia coli with HeLa cells results in translocation of cortactin to the bacterial adhertence site., Infect.Immun. 68, 382-386.

Celli, J., Olivier, M. & Finlay, B. B. (2001) Enterophathogenic Escherichia coli mediates antiphagocytosis through the inhibition of PI 3-kinase-dependent pathways., EMBO J. 20, 1245-1258.

de Grado, M., Abe, A., Gauthier, A., Steele-Mortimer, O., DeVinney, R. & Finlay, B. B. (1999) Identification of the intimin-binding domain of Tir of enteropathogenic Escherichia coli., Cellular Microbiol. 1, 7-17.

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Freeman, N. L., Zurawski, D. V., Chowrashi, P., Ayoob, J. C., Huang, L., Mittal, B., Sanger, J. M. & Sanger, J. W. (2000) Interaction of the enteropathognic Escherichia coli protein, translocated intimin receptor (Tir), with focal adhesion proteins., Cell Motility Cytoskeleton. 47, 307-318.

Gansheroff, L. J. & O'Brien, A. D. (2000) Escherichia coli 0157:H7 in beef cattle presented for slaughter in the U.S.: Higher prevelance rates than previously estimated., PNAS. 97, 2959-2961.

Goosney, D. L., Celli, J., Kenny, B. & Finlay, B. B. (1999) Enteropathogenic Escherichia coli inhibits phagocytosis., Infect.Immun. 67, 490-495.

Goosney, D. L., de Grado, M. & Finlay, B. B. (1999) Putting E.coli on a pedistal: a unique system to study signal transduction and the actin cytoskeleton., Trends Cell Biol. 9, 11-14. 

Heuvelink, A. E., van den Biggelaar, F. L. A. M., Zwartkruis-Nahuis, J. T. M., Herbes, R. G., Huyben, R., Nagelkerke, N., Melchers, W. J. G., Monnens, L. A. H. & de Boer, E. (1998) Occurence of verocytotoxin-producing Escherichia coli 0157 on dutch dairy farms, J.Clin.Microbiol. 36, 3480-3487.

Kalman, D., Weiner, O. D., Goosney, D. L., Sedat, J. W., Finlay, B. B., Abo, A. & Bishop, J. M. (1999) Enteropathogenic E.coli acts through WASP and Arp2/3 complex to form actin pedestals., Nature Cell Biol. 1, 389-391.

Knutton, S., Rosenshine, I., Pallen, M. J., Nisan, I., Neves, B. C., Bain, C., Wolff, C., Dougan, G. & Frankel, G. (1998) A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epthelial cells., EMBO J. 17, 2166-2176.

Luo, Y., Frey, E. A., Pfuetzner, R. A., Creagh, A. L., Knoechel, D. G., Haynes, C. A., Finlay, B. B. & Strynadka, N. C. J. (2000) Crystal structure of enteropathogenic Escherichia coli intimin-receptor complex., Nature. 405, 1073-1077.

Manjarrez-Hernandez, A., Gavilanes-Parra, S., Chavez-Berrocal, M. E., Molina-Lopez, J. & Cravioto, A. (1997) Binding of diarrheagenic Escherichia coli to 32- to 33-Kilodalton human intestinal brush border proteins., Infect.Immun. 65, 4494-4501.

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Matthews, K. R., Murdough, P. A. & Bramley, A. J. (1997) Invasion of bovine epithelial cells by verocytotoxin- producing Escherichia coli 0157:H7, J.Appl.Microbiol. 82, 197-203.

Mulvey, M. A., Lopez-Boado, Y. S., Wilson, C. L., Roth, R., Parks, W. C., Heuser, J. & Hultgren, S. J. (1998) Induction and evasion of host defenses by type 1-pileated urophathogenic Escherichia coli. Science. 282, 1494-1497.

Mulvey, M. A., Schilling, J. D., Martinez, J. J. & Hultgren, S. J. (2000) Bad bugs and beleaguered bladders: Interplay between uropathogenic Escherichia coli and innate host defense., PNAS. 97, 8829-8835.

Mulvey, M. A. (2002) Adhesion and entry of uropathogenic Escherichia coli. Cellular Microbiol. 4, 257-271.

Nataro, J. P. & Kaper, J. B. (1998) Diarrheagenic Escherichia coli, Clin.Microbiol.Rev. 11, 142-201.

Paton, A. W., Morona, R. & Paton, J. C. (2000) A new biological agent for treatment of shiga toxigenic Escherichia coli infections and dysentry in humans., Nature Medicine. 6, 265-270.

Paton, A. W., Voss, E., Manning, P. A. & Paton, J. C. (1998) Antibodies to lipopolysaccharide block adherence of shiga toxin-producing Escherichia coli to human intestinal epithelial (Henle 407) cells., Microbial Pathogenesis. 24, 57-63.

Peiffer, I., Servin, A. L. & Bernet_Camard, M.-F. (1998) Piracy of decay-accelerating factor (CD55) signal transduction by the diffusely adhering strain Escherichia coli C1845 promotes cytoskeletal F-actin reaggangements in cultured human intestinal INT407 cells., Infect.Immun. 66, 4036-4042.

Pennington, T. H. (1997) Death in Wishaw 1996- E.coli 0157 and food safety, 2nd Opinion. 8, 10-11.

Pichoff, S. & Lutkenhaus, J. (2002) Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli., EMBO J. 21, 685-693.

Pruimboom-Brees, I. M., Morgan, T. W., Ackermann, M. R., Nystrom, E. D., Samuel, J. E., Cornick, N. A. & Moon, H. W. (2000) Cattle lack vascular receptors for Escherichia coli 0157:H7 Shiga toxins., PNAS. 97, 10325-10329.

Schmidt, G., Sehr, P., Wilm, M., Selzer, J., Mann, M. & Aktories, K. (1997) Gln63 of Rho is deaminated by Escherichia coli cytotoxic necrotizing factor, Nature. 387, 725-729.

56. Sibille, I., Sime-Ngando, T., Mathieu, L. & Block, J. C. (1998) Protozoan bacterivory and Escherichia coli survival in drinking water distribution systems, App.Environ.Microbiol. 64, 197-202.

Sinclair, J. F. & O'Brien, A. D. (2002) Cell surface-localized nucleolin is a eukaryotic receptor for the adhesin intimin-g of enterohemorrhagic Escherichia coli 0157:H7., J.Biol.Chem. 277, 2876-2885.

Stömberg, N., Marklund, B.-I., Lund, B., Ilver, D., Hamers, A., Gaastra, W., Karlsson, K.-A. & Normark, S. (1990) Host-specificity of urophathogenic Escherichia coli depends on differences in binding specificity to Gala1-4Gal-containing isoreceptors, EMBO J. 9, 2001-2010.

Taylor, K. A., O'Connell, C. B., Luther, P. W. & Donnenberg, M. S. (1998) The EspB protein of enteropathogenic Escherichia coli is targeted to the cytoplasm of infected HeLa cells., Infect.Immun. 66, 5501-5507.

Vallance, B. A. & Finlay, B. B. (2000) Expoitation of host cells by enteropathogenic Escherichia coli., PNAS. 97, 8799-8806.

Zobiack, N., Rescher, U., Laarmann, S., Michgehl, S., Schmidt, M. A. & Gerke, V. (115) Cell-surface attachment of pedestal-forming enteropathogenic E.coli induces a clustering of raft components and a recruitment of annexin 2., J. Cell Sci. 115, 91-98.

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