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Pleckstrin Homology domains

Pleckstrin Homology (PH) domains are particular motifs that can be identified by sequence analysis that bind a variety of ligands despite sharing a similar fold.  Pleckstrin, the protein for which the domain was named is a cytoskeletal protein Many proteins with a wide variety of functions contain PH domains (table1 )

Name

 

Function

 

Ligand bound by PH domain

 

References

 

ADP-ribosylation factor (ARF)  
Bruton's tyrosine kinase (BTK) A cytoplasmic tyrosine kinase PIP2, PIP3, F-actin,  PKC Yao et al, 1994;Yao et al, 1999
Dual adaptor for phosphotyrosine and 3-phosphoinositides (DAPP1)
General receptor for PIs (GRP1) Klarlund et al, 2000
Tandem PH-domain containing protein 1 (TAPP1)
 Blomberg's PH pages  Pawson Lab PH page    Other Protein Domains

References:-

Dowler, S., Currie, R. A., Campbell, D. G., Deak, M., Kular, G., Downes, C. P. & Alessi, D. R. (2000) Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities., Biochem.J. 351, 19-31.

Hu, M. H., Bauman, E. M., Roll, R. L., Yeilding, N. & Abrams, C. S. (1999) Pleckstrin 2, a widely expressed paralog of pleckstrin involved in actin rearrangement., J.Biol.Chem. 274, 21515-21518.

Klarlund, J.K., Tsiaras, W., Holik, J.J., Chawla, A. & Czech, M.P. (2000). "Distinct polyphosphoinositde binding selectivities for pleckstrin homology domains of GRP1-like proteins based on diglycine versus triglycine motifs." J.Biol.Chem. 275(42), 32816-32821

Lemmon, M. A. & Ferguson, K. M. (2000) Signal-dependent membrane targeting by pleckstrin homology (PH) domains., Biochem. J. 350, 1-18.

Ma, A. D. & Abrams, C. S. (1999) Pleckstrin induces cytoskeletal reorganization via a Rac-dependent pathway., J.Biol.Chem. 274, 28730-28735.

Maroun, C. R., Moscatello, D. K., Naujokas, M. A., Holgado-Madruga, M., Wong, A. J. & Park, M. (1999) A conserved inositol phospholipid binding site within the plackstrin homology domain of the Gab1 docking protein is required for epithelial morphogenesis., J.Biol.Chem. 274, 31719-31726.

Peyrollier, K., Hajduch, E., Gray, A., Litherland, G. J., Prescott, A. R., Leslie, N. R. & Hundal, H. S. (2000) A role for the actin cytoskeleton in the hormonal and growth-factor-mediated activation of protein kinase B., Biochem.J. 352, 617-622.

Thomas, C. C., Dowler, S., Deak, M., Alessi, D. R. & van Aalten, D. M. F. (2001) Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding plckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity., Biochem. J. 358, 287-294.

Yao, L., Kawakami, Y. and Kawakami, T. (1994) PNAS 91, 9175-9179.

Yao, L., Janmey, P., Frigeri, L. G., Han, W., Fujita, J., Kawakami, Y., Apgar, J. R. & Kawakami, T. (1999) Pleckstrin homology domains interact with filamentous actin., J.Biol.Chem. 274, 19752-19761.

 
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