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The Golgi Cytoskeleton

Although the Golgi apparatus is best known to be associated with microtubules (reviewed Thyberg & Moskalewski, 1999), actin and many actin binding proteins are known to be associated with the Golgi body (Valderrama et al, 1998), and disruption of the actin cytoskeleton (for example by C2 Clostridium botulinum toxin) causes a concomitant collapse of the Golgi (Valderrama et al, 2000).  Some of these ABPs are present as Golgi-specific isoforms (Table 1), and many (e.g. myosins, filamins) are involved in directional trafficking whilst others may have a role in structure.  

Actin Binding Protein Function Reference
Abp1p An SH3 and ADFH containing ABP Fucini et al, 2002
ABP280 Filamin An ABP with ability to bind membranes. Liu et al, 1997
Ankyrin Not an ABP directly but anchors spectrin to the Golgi membrane. Present as a Golgi specific isoform. Beck et al, 1987
Drebrin Bundles microfilaments and is associated with a distinct actin binding complex Fucini  et al, 2000
IQGAP A signalling ABP McCallum et al, 1998; 1999
Profilin I A PIP2 and ABP with signalling involvement Dong et al, 2000
Myosin II Motor protein  Stow & Heimann, 1998
Myosin VI A minus directed actin based motor protein Buss et al, 1998
Spectrin Membrane attached ABP that binds many other proteins Fath et al, 1997; Beck & Nelson, 1998
Tm5 Tropomyosin Microfilament stabilization, regulation. Heimann et al, 1999
Table 1 Golgi associated Actin Binding Proteins

ADP-ribosylation factors and Golgi regulation.
ADP-ribosylation factors (ARFs) regulated vesicle transport (
Mellman & Warren, 2000; Fucini et al, 2000) and actin. ARF regulates the association of spectrin with the Golgi membrane (Godi et al, 1998), and the association of actin to the Golgi via Rho family G-protein localization (Rac, CDC42, but not Rho itself) (Erickson et al, 1996;Fucini et al, 2002;Wu et al, 2000) and WASP/Arp2/3 complex (Fucini et al, 2002).

The Golgi Apparatus

 

 

References:-

Beck, K.A., Buchanan, J.A. & Nelson, W.J. (1987). "Golgi membrane skeleton: identification, localization and oligomerization of a 195 kDA ankyrin isoform associated with the Golgi complex." J.Cell Sci. 110, 1239-1249.

Beck, K.A. & Nelson, W.J. (1998). "A spectrin membrane skeleton of the Golgi complex." Biochem.Biophys.Acta. 1404, 153-160.

Buss, F., Kendrick-Jones, J., (Lionne, C., Knight, A.E., Cote, G.P. & Luzio, J.P. (1998). “The localization of myosin VI at the Golgi complex and leading edge of fibroblasts and its phosphorylation and recruitment into membrane ruffles of A431 cells after growth factor stimulation.".” J.Cell Biol. 143(6), 1535-1545.

Dong, J., Radau, B., Otto, A., Muller, E.C., Lindschau, C. & Westermann, P. (2000). “Profilin I attached to the Golgi is required or the formation of constitutive transport vesicles at the trans-Golgi network.” Biochim.Biophys.Acta 1497, 253-260.

Erickson, J.W., Zhang, C., Khan, R.A., Evans, T. & Cerione, R.A. (1996). Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus. J.Biol.Chem. 271, 26850-26854.

Fath, K. R., G. M. Trimbur, et al. (1997). “Molecular motors and a spectrin matrix associate with golgi membranes in vitro.” J.Cell Biol. 139: 1169-1181

Fucini, R. V., Navarrete, A., Vadakkan, C., Lacomis, L., Erdjument-Bromage, H., Tempst, P. & Stamnes, M. (2000) Activated ADP-ribosylation factor assembles distinct pools of actin on Golgi membranes., J.Biol.Chem. 275, 18824-18829.

Fucini, R. V., Chen, J.-L., Sharma, C., Kessels, M. M. & Stamnes, M. (2002) Golgi vesicle proteins are linked to the assembly of an actin complex defined by mAbp1. Mol.Biol. Cell. 13, 621-631.

Godi, A. et al (1998) ADP ribosylation factor regulates spectrin binding to the Golgi complex. PNAS, 95, 8607-8612

Gordon, V.M., Klimpel, K.R., Arora, N., Henderson, M.A., & Leppla, S.H. (1995). "Proteolytic activation of bacterial toxins by eukaryotics cells is performed by furin and by additional cellular proteases." Infect.Immun. 63, 82-87.

Heimann, K., Percival, J.M., Weinberger, R., Gunning, P. & Stow, J.L. (1999). "Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles." J.Biol.Chem. 274, 10743-10750.

Lippincott-Schwartz (1998). "Cytoskeletal proteins and Golgi dynamics". Curr.Op.Cell Biol. 10, 52-59.

Liu, G., Thomas, L., Warren, R.A., Enns, C.A., Cunningham, C.C., Hartwig, J.H. & Thomas, G. (1997). "Cytoskeletal protein ABP-280 directs the intracellular trafficking of furin and modulates proprotein processing in the endocytic pathway." J.Cell Biol. 139, 1719-1733.

McCallum, S. J., Erickson, J.W. & Cerione, R.A. (1998). “Characterization of the association of the actin-binding protein, IQGAP, and activated Cdc42 with Golgi membranes.” J.Biol.Chem. 273(35), 22537-22544.

Mellman, I. & Warren, G. (2000). The road taken: past and future foundations of membrane traffic. Cell 100, 99-112.

Stow, J. L. & Heimann, K.  (1998). “Vesicle budding on Golgi membranes: regulation by G proteins and myosin motors.” Biochim.Biophys.Acta. 1404, 161-171.

Thymerg, J. & Moskalewski, S. (1999) Role of microtubules in the organization of the golgi complex, Exp.Cell Res. 246, 263-279.

Valderrama, F., Luna, A., Babia, T., Martinez-Menarquez, J. A., Ballesta, J., Barth, H., Chaponnier, C., Renau-Piqueras, J. & Egea, G. (2000) The golgi-associated COPI-coated buds and vesicles contain b/g-actin. PNAS. 97, 1560-1565.

Wu, W.J., Erickson, J.W., Lin, R., & Cerione, R.A. (2000). The gamma-subunit of the coatomer complex binds Cdc42 to mediate transformation. Nature 405, 800-804.

 
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