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Nuclear Actin

page updated 20/5/03

The question of the existence of actin in the nucleus has been a contentious one. High concentrations of cytosolic actin meant that biochemical identification of actin in the nuclei is questionable due to inevitable contamination, while the density of nuclei was cited as a reason to doubt nuclear localization by  immunofluorescence .  However, data has gradually become available to suggest that actin is present in the nucleus where it performs important roles in nucleosome remodeling, transcription and structure.  
Nuclear actin and ribonucleoprotein complexes.
Piergiorgio Percipalle's Lab Nuclear Actin Nuclear Actin-Binding Proteins
 

References:-

Ankenbauer, T., Kleinschmidt, J. A., Walsh, M. J., Weiner, O. H. & Franke, W. W. (1989) Identification of a widespread nuclear actin binding protein. Nature. 342, 822-825.

Gonsior, S. M., Platz, S., Buchmeier, S., Scheer, U., Jockusch, B. M. & Hinssen, H. (1999) Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J.Cell Sci. 112, 797-809.

Percipalle, P., Zhao, J., Pope, B., Weeds, A., Lindberg, U. and Daneholt, B. (2001). Actin bound to the hnRNP protein hrp36 is associated with Balbiani ring mRNA from the gene to polysomes. J. Cell Biol. 153, 229-235.

Percipalle, P., Fomproix, N., Kylberg, K., Miralles, F., Bjorkroth, B., Daneholt, B. & Visa, N. (2003) An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II. PNAS, 1131933100.

Posern, G., Sotiropoulos, A. & Treisman, R. (2002) Mutant Actins Demonstrate a Role for Unpolymerized Actin in Control of Transcription by Serum Response Factor. Mol. Biol. Cell. 13, 4167-4178.

Prendergast, G. C. & Ziff, E. B. (1991) Mbh1: a novel gelsolin/severin-related protein which binds actin in vitro and exhibits nuclear localization in vivo. EMBO Journal. 10, 757-766.

Miralles, F., Posern, G., Zaromytidou, A.-I. & Treisman, J. E. (2003) Actin dynamics control SRF activity by regulation of its coactivator MAL. Cell. 113, 329-342.

Nowak, G., Pestic-Dragovich, L., Hozak, P., Philimonenko, A., Simerly, C., Schatten, G. & de Lanerolle, P. (1997) Evidence for the Presence of Myosin I in the Nucleus. J. Biol. Chem. 272, 17176-17181.

Skare, P., Kreivi, J. P., Bergstrom, A. & Karlsson, R. (2003) Profilin I colocalizes with speckles and Cajal bodies: a possible role in pre-mRNA splicing. Exp.Cell Res. 286, 12-21.

Wada, A., Fukuda, M., Mishima, M. & Nishida, E. (1998) Nuclear export of actin; a novel mechanism regulating the subcellular localization of a major cytoskeletal protein. EMBO J. 17, 1635-1641.

Wulfkuhle, J. D., Donina, I. E., Stark, N. H., Pope, R. K., Pestonjamasp, K. N., Niswonger, M. L. & Luna, E. J. (1999) Domain analyis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals. J.Cell Sci. 112, 2125-2136.

Zhang, S., Buder, K., Burkhardt, C., Schlott, B., Gorlach, M. & Grosse, F. (2002) Nuclear DNA helicase II/RNA helicase A binds to filamentous actin., J.Biol.Chem. 277, 843-853.

 
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