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Abp1p (SH3P7, HIP-55)
Originally identified in yeast, as an actin binding protein (Drubin et al, 1990) associated with the membrane (Mulholland et al, 1994), Abp1p has an ADF homology domain and an SH3 domain.  Mammalian homologs have been discovered and are thought to have a role in endocytosis (Kessels et al, 2000 & 2001).  Although not an essential gene in itself (Holtzman et al, 1993; Adams et al, 193), Abp1p is required in fimbrin nulls and SLA1 SLA2 nulls. Abp1p binds actin through a region homologous to ADF/cofilin and to dynamin through the SH3 domain (Kessels et al, 2001).  It is possibly through the interaction of dynamin that Abp1p may have a function to play in the complex process of endocytosis (Jeng & Welsch, 2001).  It is surprising that Abp1p is not an essential gene (in yeast) as this protein seems to be involved in so many different many actin -dependent activities.

A number of binding partners for the SH3 domain and other domains have now been established (Table 1).

Protein Function Abp1p domain bound References
Actin Forms filaments ADH domain  
Ark1p Actin regulating kinase SH3 Fazi et al, 2002
Arp2/3 complex Nucleates actin filaments and caps pointed ends of filaments.   Goode et al, 2001; Evangelista,  et al, 2000
Myosin V Actin based motor SH3 Fazi et al, 2002
Prk1p Actin regulating kinase SH3 Fazi et al, 2002
RVS167   SH3 Lila & Drubin, 1997
Srv2p CAP An adenylyl cyclase associated protein that activates its activity which itself is an actin-binding protein. SH3 Lila & Drubin, 1997
Synaptojanin   SH3 Fazi et al, 2002
       
Table 1      

References:-

Adams, A. E. M., Cooper, J. A. & Drubin, D. G. (1993) Unexpected combinations of null mutations in genes encoding the actin cytoskeleton are lethal in yeast, Mol.Biol.Cell. 4, 459-468.

Drubin, D. G., Mulholland, J., Zhu, Z. & Botstein, D. (1990) Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I, Nature. 343, 288-290.

Evangelista, M.

Fazi, B., Cope, M. J. T. V., Douangamathi, A., Ferracuti, S., Schirwitz, K., Zucconi, A., Drubin, D. G., Wilmanns, M., Cesareni, G. & Castagnoli, L. (2002) Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1., J.Biol.Chem. 277, 5290-5298.

Goode, B. L., Rodal, A. A., Barnes, G. & Drubin, D. G. (2001) Activation of the Arp2/3 complex by the actin filament binding protein Abp1p., JCB. 153, 627-634.

Holtzman, D. A., Yang, S. & Drubin, D. G. (1993) Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae, J.Cell Biol. 122, 635-644.

Jeng, R.L. & Welsh, M.D. (2001) Cytoskeleton: Actin and endocytosis- no longer the weakest link. Current Biology 11, R691-R694

Kessels, M. M., Engqvist-Goldstein, A. E. Y., Drubin, D. G. & Qualmann, B. (2001) Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin., J.Cell Biol. 153, 351-366.

Kessels, M. M., Engqvist-Goldstein, A. E. Y. & Drubin, D. G. (2000) Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src Kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation., Mol.Biol.Cell. 11, 393-412.

Lila, T. & Drubin, D.G. (1997). Evidence for physical and functional interactions amongst two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton Mol.Biol.Cell 8, 367-385.

Mulholland, J., Preuss, D., Moon, A., Wong, A., Drubin, D. & Botstein, D. (1994) Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane, J Cell Biol. 125, 381-91.

Olazabal, I. M. & Machesky, L. M. (2001) Abp1p and cortactin, new "hand holds" for actin., J.Cell Biol. 154, 679-682.

Yang, H.-C. & Pon, L. A. (2002) Actin cable dynamics in budding yeast., PNAS. 99, 751-756.

 
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