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ActA

A protein expressed by the bacterium Listeria monocytogenes that is responsible for the "rocketing" motility of the bacterium throughout the eukaryotic host cell.  In addition to other host proteins (Table 1), ActA binds actin  directly (Cicchetti et al, 1999). 

References:-

Brundage, R. A., Smith, G. A., Camilli, A., Theriot, J. A. & Portnoy, D. A. (1993) Expression and phosphorylation of the Listeria-monocytogenes Acta protein in mammalian-cells, Proc. Natl. Acad. Sci. 90, 11890-11894.

Cicchetti, G., Maurer, P., Wagener, P. & Kocks, C. (1999) Actin and phosphoinositide binding by the ActA protein of the bacterial pathogen Listeria monocutogenes., J.Biol.Chem. 274, 33616-33626.

Friederich, E., Gouin, E., Hellio, R., Kocks, C., Cossart, P. & Louvard, D. (1995) Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function, EMBO J. 14, 2731-2744.

Gouin, E., Dehoux, P., Mengaud, J., Kocks, C. & Cossart, P. (1995) IactA of Listeria ivanovii, although distantly related to Listeria monocytogenes actA, restores actin tail formation in an L. monocytogenes actA mutant, Infection & Immunity. 63, 2729-2737.

Goossens, P. L. & Milon, G. (1992) Induction of protective cd8+ lymphocytes-t by an attenuated listeria-monocytogenes-acta mutant, International Immunology. 4, 1413-1418.

Kocks, C. & Cossart, P. (1993) Directional actin assembly by Listeria-monocytogenes at the site of polar surface expression of the acta gene-product invllving the actin-bundling protein plastin (fimbrin), Infectious Agents And Disease Reviews Issues And Commentary. 2, 207-209.

Kocks, C., Hellio, R., Gounon, P., Ohayon, H. & Cossart, P. (1993) Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly, J.Cell Sci. 105, 699-710.

Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H. & Cossart, P. (1992) L.monocytogenes-induced actin assembly requires the actA gene-product, a surface protein, Cell. 68, 521-531.

Kocks, C., Marchand, J. B., Gouin, E., D'hauteville, H., Sansonetti, P. J., Carlier, M. F. & Cossart, P. (1995) The unrelated surface-proteins ActA of Listeria-monocytogenes and icsa of Shigella-flexneri are sufficient to confer actin-based motility on Listeria-innocua and Escherichia-coli respectively, Molecular Microbiology. 18, 413-423.

Kreft, J., Dumbsky, M. & Theiss, S. (1995) The actin-polymerization protein from Listeria-ivanovii is a large repeat protein which shows only limited amino-acid-sequence homology to ActA from Listeria-monocytogenes., FEMS Letters. 126, 113-121.

Lasa, I., Gouin, E., Goethals, M., Vancampernolle, K., David, V., Vandekerckhove, J. & Cossart, P. (1997) Identifiaction of two regions in the N-terminal domain of ActA involved in the actin comet tail formation by Listeria monocytogenes, EMBO J. 16, 1531-1540.

Lasa, I., David, V., Gouin, E., Marchand, J. B. & Cossart, P. (1995) The amino-terminal part of ActA is critical for the actin-based motility of Listeria-monocytogenes - the central proline-rich region acts as a stimulator, Molecular Microbiology. 18, 425-436.

Mourrain, P., Lasa, I., Gautreau, A., Gouin, E., Pugsley, A. & Cossart, P. (1997) ActA ia a dimer, Proc.Nat.Acad.Sci.USA. 94, 10034-10039.

Niebuhr, K., Chakraborty, T., Rohde, M., Gazlig, T., Jansen, B., Kollner, P. & wehland, J. (1993) Localization of the ActA polypeptide of Listeria - monocytogenes in infected tissue-culture cell-lines - ActA is not associated with actin comets, Infection And Immunity. 61, 2793-2802.

Niebuhr, K., Ebel, F., Frank, R., Reinhard, M., Domann, E., Carl, U. D., Walter, U., Gertler, F. B., Wehland, J. & Chakraborty, T. (1997) A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family, EMBO J. 16, 5433-5444.

Pistor, S., Chakraborty, T., Niebuhr, K., Domann, E. & Wehland, J. (1994) The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton, EMBO J. 13, 758-763.

Pistor, S., Chakroborty, T., Walter, U. & Wehland, J. (1995) The bacterial actin nucleator protein ActA of Listeria monocytogenes contain multiple binding sites for host microfilament proteins, Current Biol. 5, 517-525.

Smith, G. A., Theriot, J. A. & Portnoy, D. A. (1996) The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of Vasodilator-stimulated phosphoprotein and profilin, J.Cell Biol. 135, 647-660.

Smith, G. A. & Portnoy, D. A. (1997) How the Listeria monocytogenes ActA protein converts actin polymerization inot a motile force, Trends Microbiol. 5, 272-276.

Southwick, F. S. & Purich, D. L. (1994) Arrest of Listeria movement in host-cells by a bacterial acta analogue: implications for actin-based motility., Proc. Natl. Acad. Sci. 91, 5168-5172.

Smith, G. A., Portnoy, D. A. & Theriot, J. A. (1995) Asymmetric distribution of the listeria-monocytogenes acta protein is required and sufficient to direct actin-based motility, Molecular Microbiology. 17, 945-951.

Welch, M. D., Rosenblatt, J., Skoble, J., Portnoy, D. A. & Mitchison, T. J. (1998) Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation, Science. 281, 105-108.

Zalevsky, J., Grigorova, I. & Mullins, R. D. (2001) Activation of the Arp2/3 complex by the Listeria ActA protein., J.Biol.Chem. 276, 3468-3475.

 

 
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