EDInfo Biomedical Sciences Maciver Lab. Home ABP  A-Z Encyclopaedia Amoebae Protist Links Cytoskeleton Links Site Index

Filamin (ABP, gyronemin).

Page updated 11/7/'02

A large (270kd) dimeric actin crosslinking protein from a variety of sources.  Filamin cross-links microfilaments into an orthogonal gel without  producing bundles, presumably because the filaments are linked at right angle to each other.  Like ABP-120, filamin has an actin binding headpiece close to the N-terminus followed by b-cross structures. Whereas in ABP-120 there are six domains, in filamin there are 24 of these structures.

Binding Partner Effect Regulated by Reference
Presenilin Guo et al, 2000
TNF receptor Leonardi et al, 2000
SEC-1 Marti et al, 1997
Ig receptor Ohta et al, 1997
Caveolin-1 Stahlhut & van Deurs, 2000
GP Iba Meyer et al, 1997
FILIP Leads to proteolyis of filamin  Not known Nagano et al, 2002
Migfilin Localises Mig-2 to focal adhesions   Tu et al, 2003
Table 1 Filamin binding proteins



Cunningham, C. C., Gorlin,  J. B., Kwiatkowski, D. J., Hartwig, J. H., Janmey, P. A., Byers, H. R. & Stossel, T. P. (1992). “Actin-binding protein requirement for cortical stability and efficient locomotion.” Science 255, 325-327.

Fox, J.W., Lamperti, E.D., Eksioglu, Y.Z., Hong, S.E., Feng, Y., Graham, D.A., Scheffer, I.E., Dobyns, W.B., Hirsch, B.A., Radtke, R.A., Berkovic, S.F., Huttenlocher, P.R. & Walsh, C.A. (1998). "Mutations in filamin 1 prevent migration of cerebral cortical neurons in human periventricular heterotopia." Neuron 21, 1315-1325.

Goldmann, W. H. and  Isenberg, G. (1993). “Analysis of filamin and a-actinin binding to actin by the stopped flow method.” FEBS letters 336(3), 408-410.

Gorlin, J., Yamin, J., Egan, S., Stewart, M., Stossel, T., Kwiatkowski, D. & Hartwig, J. (1990). "Human endothelial actin-binding protein (ABP-280, nonmuscle filamin): a molecular leafspring." J.Cell Biol. 111, 1089-1105.

Gorlin, J. B., E. Henske, E., Warren, S. T., Kunst, C. B., Durso, M., Palmieri, G., Hartwig, J. H., Bruns, G. & Kwiatkowski, D. J. (1993). “Actin-binding protein (abp-280) filamin gene (fln) maps telomeric to the color-vision locus (r/gcp) and centromeric to g6pd in xq28.” Genomics: 496-498.

Guo, Y., Zhang, S.X., Sokol, N., Cooley, L.& Boulianne, G.L.(2000)."Physical and genetic interaction of filamin with presenilin in Drosophila." J.Cell Sci. 113, 3499-3508.

Leedman, P. J., B. Faulkner-Jones, B., Cram, D.S., Harrison, P.J., West, J., O'Brien, E., Simpson, R., Coppel, R.L., & Harrison, L.C. (1993). “Cloning from the thyroid of a protein related to actin binding protein that is recognized by Graves disease immunoglobulins.” PNAS.USA 90, 5994-5998.

Lebart, M. C., C. Méjean, C., Casanova, D., Audemard, E., Derancourt, J., Roustan, C.& Benyamin, Y.(1994). “Characterization of the actin binding site on smooth muscle filamin.” J. Biol. Chem. 269, 4279-4284.

Leonardi, A., H. Ellinger-Ziegelbauer, H., Franzoso, G., Brown, K. & Siebenlist, U. (2000). “Physical and functional interaction of filamin (actin-binding protein 280) and tumor necrosis factor receptor-assoicated factor 2.J.Biol.Chem. 275(1), 271-278 

Li, M.-g., Serr, M., Edards, K., Ludmann, S., Yamamotot, D., Tilney, L.G., Field, C.M. & Hays, T.S. (1999). " Filamin is required for ring canal assembly and actin organization during Drosophila oogenesis." J.Cell Biol. 146(5), 1061-1074.

Liu, Y.-M., Thomas, L., Warren, R.A., Enns, C.A., Cunningham, C.C., Hartwig, J.H. & Tomas, G. (1997). "Cytoskeletal protein ABP-280 directs the intramolecular trafficing of furin and modulates proprotein processing in the endocytic pathway." J.Cell Biol. 139, 1719-1733.

Marti, A., Z. Luo, Z., Cunningham, C., Ohta, Y., Hartwig, J., Stossel, T.P., Kyriakis, J.M. & Avruch, J. (1997). “Actin -binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-a activation of SAPK in melanoma cells.” J.Biol.Chem. 272(5), 2620-2628.

Méjean, C.,  Lebart, M.C., Poyer, M., Roustan, C. & Benyamin, Y. (1992). “Localization and identification of actin structures involved in the filamin-actin interaction.” Eur.J.Biochem. 209, 555-562.

Meyer, S.C., Zuerbig, S., Cunningham, C.C., Hartwig, J.H., Bissell, T., Gardner, K. & Fox, J.E.B.(1997). "Identification of the region in actin-binding protein that binds to the cytoplasmic domain of glycoprotein Iba." J.Biol.Chem. 272, 2914-2919

Nagano, T., Yoneda, T., Hatanaka, Y., Kubota, C., Murakami, F. & Sato, M. (2002) Filamin A-interacting protein (FILIP) regulates cortical cell migration out of the ventricular zone. Nature Cell Biol. 4, 495-501.

Ohta, Y., N. Suzuki, N. Nakamura, S., Hartwig, J.H. & Stossel, T.P. (1999). The small GTPase Ral1 targets filamin to induce filopodia. PNAS USA 96, 2122-2128.

 Ohta, Y., T. P. Stossel, T., & Hartwig, J.H. (1991). “Ligand-sensitive binding of actin-binding protein to immunoglobulin G Fc receptor i (FcgR1).” Cell 67, 275-282.

Stahlhut, M. and B. van Deurs (2000). “Identification of filamin as a novel ligand for caveolin-1: Evidence for the organization of caveolin-1-associated membrane domains by the actin cytoskeleton.” Mol.Biol.Cell 11, 325-337.

Tu, Y., Wu, S., Shi, X., Chen, K. & Wu, C. (2003) Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation. Cell. 113, 37-47.

  EDInfo Biomedical Sciences Cytoskeletal Links Encyclopaedia of A.B.P.s The Amoebae Protozoology links Glossary of Amoeba terms   Maciver Lab Home