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Transgelin (SM22)

A 23kDa Calponin-related protein (Shapland et al, 1988; Prinjha et al, 1994) found in smooth muscle and non-muscle cells.  The protein binds actin in vitro only if the salt concentration is low, however transgelin co-localises with F-actin in cells by immunofluorescence microscopy (Shapland et al, 1988), indicating that perhaps another factor influences actin binding in vivo.  The expression of this protein is transformation sensitive and is lost in a variety of cell types upon transformation, possibly through the deregulation of the Ras pathway.  However, no decrease in the transformed phenotype was seen as the transgelin was re-expressed in the transformed cells (Shields et al, 2002). This suggests that transgelin itself is not vitally important in the transformation process but is perhaps a "side show".

References:-

Lawson, D., Harrison, M., and Shapland, C. (1997). Cell Mot.Cytoskel. 38, 250-257.

Prinjha, R. K., Shapland, C. E., Hsuan, J. J., Totty, N. F., Mason, I. J. & Lawson, D. (1994) Cloning and sequencing of cDNAs encoding the actin cross-linking protein transgelin defines a new family of actin-associated proteins, Cell Motility and the Cytoskeleton. 28, 243-255.

Shapland, C., Lowings, P. & Lawson, D. (1988) Identification of new actin-associated polypeptides that are modified by viral transformation and changes in cell shape. J.Cell Biol. 107, 153-161.

Shapland, C., Hsuan, J. J., Totty, N. F. & Lawson, D. (1993) Purification and properties of transgelin: A transformation and shape change sensitive actin-gelling protein., J.Cell Biol. 121, 1065-1073.

Shields, J. M., Roger-Graham, K. & Der, C. J. (2002) Loss of transgelin in breast and colon tumors and in RIE-1 cells by Ras deregulation of gene expression through Raf-independent pathways. J.Biol.Chem. 277, 9790-9799.

 
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