23kDa Calponin-related protein
(Shapland et al,
et al, 1994) found in smooth muscle and non-muscle cells. The protein
binds actin in vitro only if the salt concentration is low, however
transgelin co-localises with F-actin in cells by immunofluorescence microscopy (Shapland
et al, 1988),
perhaps another factor influences actin binding in vivo. The
expression of this protein is transformation sensitive and is lost in a variety
of cell types upon transformation, possibly through the deregulation of the Ras
pathway. However, no decrease in the transformed phenotype was seen as the
transgelin was re-expressed in the transformed cells (Shields
et al, 2002).
This suggests that transgelin itself is not vitally important in the
transformation process but is perhaps a "side show".
D., Harrison, M., and Shapland, C. (1997). Cell Mot.Cytoskel. 38,
Prinjha, R. K., Shapland, C. E., Hsuan, J. J.,
Totty, N. F., Mason, I. J. & Lawson, D. (1994) Cloning and
sequencing of cDNAs encoding the actin cross-linking protein transgelin
defines a new family of actin-associated proteins, Cell Motility and
the Cytoskeleton. 28, 243-255.
Shapland, C., Lowings, P. &
Lawson, D. (1988) Identification of new actin-associated polypeptides
that are modified by viral transformation and changes in cell shape. J.Cell
Biol. 107, 153-161.
Shapland, C., Hsuan, J. J., Totty, N. F. &
Lawson, D. (1993) Purification and properties of transgelin: A
transformation and shape change sensitive actin-gelling protein., J.Cell
Biol. 121, 1065-1073.
Shields, J. M., Roger-Graham, K. &
Der, C. J. (2002) Loss of transgelin in breast and colon tumors and in
RIE-1 cells by Ras deregulation of gene expression through Raf-independent
pathways. J.Biol.Chem. 277, 9790-9799.