EDInfo Biomedical Sciences Maciver Lab. Home ABP  A-Z Encyclopaedia Amoebae Protist Links Cytoskeleton Links Site Index

Culturing of Amoeba 

Once amoeba have been obtained, either by direct isolation from environments (Amoeba isolation), or form commercial or other cultures it can be very important to establish optimum culture conditions immediately.  Many amoeba are able to form resilient cysts and so can be ignored and abused with comparative impunity! In general, the smaller the amoeba, the less trouble they are to maintain!  The so called giant amoeba (species belonging to the genera, Amoeba, Chaos, Pelomyxa) require constant care, feeding and general sub-culturing, whereas an amoeboflagellate SLB has been recovered on dried salty plates three years old as this amoeba forms very tough cysts!

A list of media follows,  the vast majority of these recipes comes from the work of others and I merely pass this information on with comment drawn from my own experience. 

 

Ingredient

Amount

Stock Soln.

Final Conc.
g/litre

NaCl

84.6 ml

5M

24.72

KCl

9.0 ml

1M

0.67

CaCl2.2H2O

9.27 ml

1M

1.36

MgCl2.6H2O

4.68 ml

4.9M

4.66

MgSO4.7H2O

12.75 ml

2M

6.29

NaHCO3

2.15 ml

1M

0.18

H2O

to 1 liter

 

-

 Table 1 Artificial Seawater

 

  Back to Maciver Lab Protocols

 

  EDInfo Biomedical Sciences Cytoskeletal Links Encyclopaedia of A.B.P.s The Amoebae Protozoology links Glossary of Amoeba terms   Maciver Lab Home