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The Dynamic Cell

Practical V

Experiment 2

 Chlamydomonas is a unicellular algae that can detect and swin toward light.  This of course helps it to maintain itself in a part of the pond that is illuminated.
Four cultures were set up (left).  Each culture was placed next to a strip light so that light could shine in through a slot in each of the cultures.  Chlamydomonas were then given the chance to swim toward the light and gather at the window (the slot).
VPA and Lithium inhibit locomotion and chemotaxis
of Dictyostelium amoebae.
VPA and Lithium inhibit phototaxis of Chlamydomonas.
Lithium inhibits inositol monophosphatase (and inositol Poly-phosphatase),
 thereby leading to a decreased inositol-1,4,5-trisphosphate
(InsP3) response.  Exact mechanism for VPA not known but it also inhibits PI
Williams, R. S. B., Cheung, L., Mudge, A. W. & Harwood, A. J. (2002) A common mechanism
of action for three mood-stabilizing drugs. Nature. 417, 292-295.


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