An Introduction to the Cytoskeleton. CS1

 

The cytoskeleton is a three dimensional network of filamentous protein which fills the space between organelles and gives shape and structure to cells.  The cytoskeleton also provides the cell with “motility”, that being the ability of the entire cell to move and for material to be moved within the cell..  Three main protein systems constitute the cytoskeleton, these are (in order of typical abundance):-  Microfilaments, Intermediate filaments and Microtubules.  Although the term “cytoskeleton” is well used and accepted it unfortunately gives an impression of a rather static entity whereas all three constituents are dynamic structures, they constantly change shape through cycles of polymerisation / depolymerisation and interactions with other proteins. 

 

Microfilaments

Microfilaments are linear assemblages of the 43 Kilodalton protein actin. Actin is the most abundant protein in typical eukaryotic cells, accounting for as much as 15% of total protein. It is a highly conserved protein: the amino-acid sequence of actin from Acanthamoeba, a small soil amoeba, is 95% identical to vertebrate isoforms of actin. X-ray crystallography has revealed that the actin monomer is approximately pear shaped, and when viewed conventionally with the more pointed end upper most, both the NH2 and the COOH termini are seen in the bottom right hand corner.

 

Figure 1  An Actin Monomer ( courtesy of Bill)

 

Actin is composed of four domains with a large cleft almost bisecting the molecule.  This cleft forms both a divalent cation (most likely magnesium in cells) and nucleotide binding site. Because the actin subunit has polarity, the microfilament also has (figure 2).  Traditionally, the ends of the microfilament have been referred to as "pointed" and "barbed".  This nomenclature arises from the resemblance of microfilaments decorated with fragments of myosin II to arrowheads in the electron microscope.  Happily, this nomenclature coincides with the pointed appearance of the actin monomer! (see figure 1 above, top is pointed end).  The microfilament is a single-stranded helix with each monomer rotated 166o with respect to neighbouring subunits which means that every 36 nm, or every 13 subunits, subunits eclipse each other at what appears to be a crossover.

 

 

Figure 2          A Microfilament

 

Microtubules

Microtubules (MTs) are assemblages of 110-kDa tubulin dimers.  Each dimer is actually a heterodimer, i.e. the polymerising subunit is one 55-kDa a-tubulin associated with one 55-kDa b-tubulin.  As their name suggests MTs are small tubes.  They are 25nm in diameter with an internal diameter of 14nm.  It is not known if materials are transported within the lumen of the MT, (this is unlikely as the ends at the cell centre are most likely blocked) so MT perform a scaffold function rather that that of a pipe.  Note that the MTs are polar, i.e. they have a b-tubulin exposed at the minus end and an a-tubulin exposed at the plus end.  Each MT is typically composed of 13 tubulins arranged around the circumference, but some MTs (especially those found in protozoans) exist which break this general rule.

 

 

Figure 3

 

 

Intermediate Filaments

Intermediate Filaments (IFs), are so called because, at 10nm in diameter they are typically intermediate in size between microfilaments and microtubules.  IFs are different to microfilaments and microtubules in a number of fundamental respects.  First of all they tend to be more or less permanent structures in tissues such as skin and hair, in fact in these non-living tissues IF proteins are almost the only protein.  Thus it is true (but a little sad) to say that beauty is only IF thick!  In other cell types, IFs are modified by phosphorylation when they are required to be disassembled for example during cell division.  Unlike the highly conserved actins and tubulins more than 40 distinct IF proteins are encoded by a number of genes in mammalian cells.  All IF proteins have a similar structure with a central helical rod domain and more variable head and tail domains.  The IFs can be divided into five major classes:-

 

Class               Name                          Tissue

 

i                       Acidic Keratins             Epithelia

ii                      Basic Keratins              Epithelia

iii                     Desmin                         Muscle

Glial                             Glial cells and astrocytes

Peripherin                     Peripheral neurones

Vimentin                       Mesenchyme

iv                     Neurofilaments             Neurons

v                      Lamins                         Nuclear envelopes

 

Figure 4.  A representation of the domain structure of the intermediate filament family.  The numbers refer to the number of amino-acids typically forming each domain.  The tail region is the most diverse, some IFs do not have any, while others (neurofilament-H), has a tail of 607 amino-acids.  IF monomers assemble in a parallel fashion:-

 

 

Dimerisation takes place by coiled-coil interaction of the a-helical domain.  The two helices (top left) associate with those of another molecule wrapping around each other (bottom left), so that the N terminus and C terminus lie next to each other.  The rectangles to the right give a simplified view for later comparison with higher order structures.

 

 

The IF dimers now associate with other dimers in an anti-parallel fashion so that there are now two N and two C termini at each end of the complex to form a tetramer (top).  The next step is the association of the N terminal head of one tetramer with the C terminal tails of another.  IF assembly can then proceed in this manner infinitely.  It should be noted that the above scheme is somewhat tentative and lacks firm evidence.  It is not clear for example exactly which domains are responsible for tetramers binding end to end.

 

Figure 5     Intermediate filament structure and assembly

 

 

Cellular Organisation of the Cytoskeleton

The three cytoskeletal components have distinct sub-cellular localisations.  Microfilaments are enriched in a layer known as the “cell cortex”, immediately beneath the plasma membrane, and in cell projections such as microvilli.  Microtubules extend from the perinucleus towards the cell periphery.  The plus ends of MTs point to the cell periphery.  IFs are distributed in a similar pattern to MTs except where cells are in contact where the IFs are enriched.  IFs and MTs are excluded from the actively expanding leading edge of the moving or “ruffling” cell.  In some situations a co-localisation of the cytoskeletal systems is seen.  For example, neurofilaments and MT co-localise in the axon of neurons where specific cross links are made between the two systems.  Cross linking proteins such as “filamin” also exist which bind both MTs and MFs.  MFs are also associated with a number of other structures in specific situations, such as the contractile ring in dividing cells, and in specific cell types such as the “focal contact” in fibroblasts,

 

 

Figure 6.         Distribution of Cytoskeletal Systems in a typical Cell

 

Thin straight lines                                  - Microfilaments.  Arranged in bundles in microvilli

Wavy thicker black lines                       - Intermediate  Filaments.  Connect cell to cell

Wavy thick grey lines                            - Microtubules.  Radiate from perinuclear MTOC.

 

 

Summary

Microfilaments and Microtubules are similar in many respects, they are polar, dynamic structures whose assembly state is nucleotide dependent and both interact with a host of associated proteins .  IFs are apolar and rather more static polymers which are depolymerised by phosphorylation.  The three systems differ in their mechanical properties; MFs form visco-elastic gels; MTs resist bending and compression; IFs are extremely tough fibres which resist stretching. MFs are arranged as gels or bundles in association with a large number of actin binding proteins.  MTs are usually single typically have their minus end associated with an MTOC deep within the cell, with the plus end toward the periphery.  IFs connect cell-cell junctions to give strength to tissues.  All three systems are interconnected to various extents.

 

References:-

 

General                   Molecular Biology of the Cell chapter 16, p787. (for third edition)

                                Bray, D. Cell Movements Garlands press (1992).

 

Intermediate filaments         Stewart, M. Current Opinion Cell Biol. 5, 3-11 (1993).

 

Please direct any questions to me at:-

Room 444 or lab 446 fourth floor Hugh Robson Building. George Square. 

Tel (0131) 650 3714 or 3712. E-mail  SKM@srv4.med.ed.ac.uk